The degree of phenotypic similarity of near-isogenic lines of the wheat cultivar Centana with their recurrent parent

Summary The degree of phenotypic similarity of Centana and its near-isogenic lines (NILs) has been investigated. Some NILs have a disease reaction, a gliadin pattern and a yield level which differs from Centana. This means that they still possess many donor genes. One of the NILs, Shortana, must be a derivative of a backcross line crossed with an unknown genotype resulting in a good yielding variety.     

Genetic diversity and relationships among pea cultivars revealed by RAPDs and AFLPs

In order to obtain an overview of the genetic diversity present within the set of pea cultivars released in Germany, 21 cultivars were analysed at the DNA level by random amplified polymorphic DNAs (RAPDs) and amplified fragment length polymorphisms (AFLPs), as well as for agronomic traits. Yield of grain cultivars ranged from 2.95 to 3.87 t/ha. Based on the screening of 60 RAPD primers and 32 Eco RI + 3/Mse I+3 AFLP primer combinations, 20 RAPD primers and 11 Eco RI + 3/MseI+ 3 primer combinations generating polymorphic and distinct fragments were chosen for estimation of genetic diversity. Twenty RAPD primers amplified a total of 314 scorable bands ranging from about 262 bp to 1996 bp. Of these, 175 fragments (55.7%) were polymorphic. Based on these data, genetic similarity (GS) was estimated between 0.80 (‘Lisa’ vs.‘Grapis’) and 0.94 (‘Bohatyr’ vs. ‘Sponsor’; mean GS = 0.88). Eleven AFLP primer combinations led to the amplification of 949 scorable fragments ranging from 43 to 805 bp and of these, 462 (48.7%) were polymorphic. Genetic similarity based on AFLPs was calculated between 0.85 (‘Lisa’ vs.‘Laser’) and 0.94 (‘Bohatyr’ vs. ‘Sponsor’, mean GS = 0.90). Correlation of genetic similarity estimated on RAPDs and AFLPs was estimated at r = 0.79** using Spearman's rank correlation coefficient and at r = 0.84 by the Mantel test, respectively. UPGMA cluster analysis carried out on these data separately for RAPDs and AFLPs and on the combined data reflected, to some extent, pedigree relationships and cophenetic correlations (r = 0.89 for RAPDs, r = 0.88 for AFLPs, and r = 0.93 RAPDs + AFLPs) indicate a good fit of respective clusters to genetic similarity data. The correlation of cluster analyses to pedigree information and the impact on parental genotype selection is discussed.     

Isozyme Banding Pattern and Estimation of Genetic Diversity among Guinea Grass Germplasm

Isozyme banding pattern was studied in Guinea grass (Panicum maximum Jacq.), a widely cultivated grass having good fodder value. Similarity among 63 accessions collected from diverse sources was worked out using five enzyme systems (SOD, GOT, ACP, Esterase and Peroxidase) following horizontal starch gel electrophoresis. Biochemical markers such as isozymes are useful supplements in identifying the genetic variation present in any crop. A total of 35 clear and unambiguous bands were used for analysis of which 8 bands were monomorphic. Polymorphism exhibited by 27 bands from all five enzyme systems indicate presence of considerable diversity in this species. The dendrogram generated after UPGMA and SAHN cluster analysis using Jaccard genetic distance showed that 63 accessions from diverse geographical locations could be grouped in three main clusters, of which two could further be divided into sub-clusters. Although the clusters comprised members from different locations, most of the accessions from similar geographical locations tended to cluster in same group.     

Species compositional similarity and ecoregions: Do ecoregion boundaries represent zones of high species turnover?

The concept of an ecoregion, a discrete spatial area where species composition is presumed to be relatively homogeneous compared to that between areas, has become an increasingly common conservation tool. We test the effectiveness of one ecoregion delineation (World Wildlife Fund) in capturing patterns of change in species composition of birds, mammals, and trees across the United States (excluding Hawaii) and Canada, and describe the extent to which each ecoregion boundary is concurrent with relatively large changes in species composition. Digitized range maps were used to record presence/absence in 50 × 50 km equal-area grid cells covering the study area. Jaccard’s index of similarity was calculated for all pairs of cells in the same or adjacent ecoregions. The average rate at which similarity declined with geographic distance was calculated using pairs of cells within the same ecoregion (intra-ecoregion turnover) or using pairs of cells in adjacent ecoregions (inter-ecoregion turnover). The intra-ecoregion rate varies widely among ecoregions and between taxa, with trees having a faster rate of similarity decline than mammals or birds. For all three species groups, most ecoregion boundaries have similar rates across them (inter-ecoregion) than that within each adjacent ecoregion (intra-ecoregion), with the exception of zones of transition between biomes and major geographical features. Although the ecoregion concept is useful for many other conservation applications, the lack of systematic, high turnover rates along ecoregion boundaries suggests that ecoregions should not be used as a quantitative basis for delineating geographic areas of a particular taxonomic group.     

基于自适应小波偏微分方程的蝗虫切片图像去噪

蝗虫显微切片图像在获取的过程中不可避免地会受到噪声污染,其纹理、边缘与噪声又都属于高频分量,单独使用小波变换或偏微分方程(partial differential equation,PDE)扩散的方法都不能在有效去噪的同时保持边缘、纹理等。针对这一问题,提出了基于自适应小波PDE的去噪算法。首先对蝗虫切片含噪图像进行sym5小波软阈值去噪,分解层数根据去噪后图像的PSNR(peak signal to noise ratio)值自适应地选择,阈值门限使用Birge-Massart处罚算法获取。然后在此去噪的基础上进行Perona-Malik(PM)模型去噪,迭代次数根据去噪后图像的PSNR值自适应地选择,梯度阈值根据图像自身的2范数获取。为了验证所提出算法的去噪性能,进行了与常用去噪算法的对比试验。试验结果表明:视觉上,采用本文算法去噪后的图像噪声点较少且边缘、纹理清晰;客观上,采用该文算法去噪后的图像PSNR值比使用维纳滤波高出2 d B左右,比使用中值滤波高出3 d B左右,比使用小波阈值去噪高出2 d B左右,比使用PM模型去噪高出1 d B左右,并且在结构相似性(structural similarity image measurement,SSIM)上采用该文算法去噪后的图像与原始图像的相似度最高。因此,将自适应小波PDE的算法应用于蝗虫切片去噪是可行的、有效的,为其后续处理提供了技术支持。     

Genetic Analysis of Egyptian Date (<Emphasis Type="Italic">Phoenix dactylifera</Emphasis> L.) Accessions Using AFLP Markers

Forty-seven samples of date palm (Phoenix dactylifera L.) collected from eight locations in Egypt were studied using four sets of amplified fragment length polymorphism (AFLP) markers with near infrared fluorescence labeled primers. These samples belonged to 21 named accessions and 9 of unknown pedigrees. A total of 350 bands were scored and 233 (66.6%) were polymorphic. Twenty-seven Egyptian accessions and ‘Medjool’and ‘Deglet Noor’accessions from California could beclassified into the major cluster. This major cluster may represent a major group of date palm germplasm in North Africa. There were four other clusters, each containing one or two accessions. The variety ‘Halawy’and one accession of unknown provenance were most likely from hybridization between two clusters. Six groups of accessions of which had the same names, revealed similar but not identical AFLP profiles suggesting these accessions might derive from seedlings rather thanthrough clonal offshoot propagation.     

油松根系形态分布的分形分析研究

基于分形理论和管状模型理论建立了油松根系的三维静态模型。根系模型主要建立在根系拓扑、分枝规律、连结长度、连结直径和根系分枝角度基础上。检测模型主要考虑参数α和q。参数α为根系分枝前后的横截面积之比,q定义分枝后新连结的生物量。虽然参数α和q相对于根系直径独立,但是它们的变化可能影响预测的正确性。在植物水平上,模型提供适当的根系干重,根系全长和根系直径的预测。这一静态模型较适合研究成熟根系,与其它模型相比,该模型的主要优点是它的可塑性和易于应用。     

ON STUDY DIGGED FIELD MOVEMENT''S LAW BY EXPERIMENTAL METHOD OF LASER HOLOGRAPHIC MOIRE INTERFERMETRY

The high-precision displacement measuring technology of optic-measuring mechanics is, for the first time,successfully applied on the similar model of the model land-layer with a new-type variable modulus of elasticity. The angle of movement and the distributed displacement of upper and lower rock-layers are measured. The technology is a combination of laser's technology and the modern chemical sticker-it is called the sticking moire interferometry. Compared with the measured values, the result is very satisfactory.     

Reasoning Method Based on Motorcycle Design

According to the needs of setting up a professional system based on motorcycle design,the CBR(case based reasoning) and RBR(rule based reasoning) methods are introduced.After discussing the difference between the two kinds of reasoning,a method of integrating them into the motorcycle design flow is put forward to realize the design reusable technology and improve the design efficiency,by using the CBR in the step of selecting the similar motorcycle and the RBR in project's modification according to correlative rules.     

Weighting Coefficient Optimization Unification of Lexicographic Method in Fuzzy Multi -objective Optimization Design

The Lexicographic method has subjective and empirical character,so a weighting coefficient is introduced by considering the importance and proportion of each individual object,the preferable result of every individual object can be confirmed by comparing the degree of similarity between the perfect one and the ecumenic one and the Fuzzy optimization will be perfect.The effectiveness of these methods is illustrated by a fuzzy optimal design of the three-bar truss.